WebNovaSeq libraries needs to have unique dual indexes Read about unique dual indexes here (opens in new tab) Pools can only be run on whole flow cells Define a sequencing kit, number of cycles and loading concentration Optimal pool loading concentration depends on the library type, insert size and is machine and sequencing kit specific WebFor the NovaSeq, average fragment length should ideally be between 420 bp and 650 bp and the “tail” of longer fragments should not exceed 1 kb. The longer library molecules will result in lower quality reads for this fraction of the samples …
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WebUltra-high density patterned flow cell. The NovaSeq X series incorporates patterned flow cell technology to generate an ultra-high level of throughput for a broad range of sequencing applications. NovaSeq X patterned flow cells contain tens of billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. Web400 NovaSeq 80.9 116.5 419.4 97.3 96.7 94.2 72.1 2 72.9 3 72.8 4 71.7 5 58.6 6 58.9 7 59.8 8 59.5 Table 3. Reported sequencing metrics for Chromium Single Cell 3’ v2 libraries on two Illumina NovaSeq instruments with recommended loading concentration. Libraries were spiked with 1% PhiX. deuter nomi backpack review
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WebChoosing a Library Loading Concentration for Illumina Sequencing Video Compatible and recommended Illumina library types for the NovaSeq 6000 sequencing platform … WebThe NovaSeq 6000 system incorporates patterned flow cell technology to generate an unprecedented level of throughput for a broad range of sequencing applications. … Webtunable output makes the NovaSeq 6000 System accessible for a wide range of applications. Maximize library loading configurations The NovaSeq 6000 System offers … church curtains ideas